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Artificial microRNA

miRNA：

miRNA Sequence：

Vector：

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Results

Cloning protocols and vectors are available in WMD3 (Web MicroRNA Designer)

Reference

Rebecca Schwab, Stephan Ossowski, Markus Riester, Norman Warthmann, and Detlef Weigel. Highly Specific Gene Silencing by Artificial MicroRNAs in Arabidopsis. The Plant Cell. 2006
Stephan Ossowski, Rebecca Schwab, Detlef Weigel. Gene silencing in plants using artificial microRNAs and other small RNAs. The Plant Journal. 2008
Warthmann N, Chen H, Ossowski S, Weigel D, Hervé P. Highly Specific Gene Silencing by Artificial miRNAs in Rice. PLoS ONE. 2008
Khraiwesh B, Ossowski S, Weigel D, Reski R, Frank W. Specific gene silencing by artificial MicroRNAs in Physcomitrella patens: an alternative to targeted gene knockouts. Plant Physiology. 2008
Molnar A, Bassett A, Thuenemann E, Schwach F, Karkare S, Ossowski S, Weigel D, Baulcombe D. Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii. The Plant Journal. 2008